Probiotic yogurt guide: Health benefits, types and recommended brands

Your complete guide to probiotic yogurts

Probiotic yogurts have become very popular thanks to their benefits for digestive health. In this article, we’ll answer the most common questions about these yogurts: What are probiotics? What benefits do they offer? What is the best probiotic yogurt? Are they suitable for children? Do they help with digestive issues like constipation or irritable bowel syndrome? And what probiotic yogurt brands are recommended?
All of this is explained in clear and accessible Language, perfect for any reader interested in improving their diet and gut health.

What are probiotics?

Probiotics are live microorganisms commonly referred to as “good bacteria” that, when consumed in adequate amounts, provide health benefits to the body. In other words, they are beneficial microbes that naturally live in our intestines and help keep them healthy. They are found naturally in fermented foods such as yogurt, kefir, sauerkraut, and certain cheeses, as well as in dietary supplements.

In the case of yogurt, it is naturally a probiotic food because during its production, milk is fermented with bacterial strains—usually Lactobacillus bulgaricus and Streptococcus thermophilus. Many yogurts are also enriched with additional probiotic strains. These bacteria survive in the intestine in sufficient quantities and help balance the intestinal microbiota, or gut flora, by competing with harmful microbes and supporting digestive health.

In short, probiotics are the friendly microorganisms found in yogurt and other fermented foods that, when ingested, can help restore the balance of gut flora and support various functions of the body.

What are the benefits of probiotic yogurts?

Consuming yogurts with probiotics on a regular basis can have multiple health benefits. By incorporating “good bacteria” into your diet, you are supporting your intestinal ecosystem, which has a positive impact on your entire body. Below, we summarize some of the main benefits of probiotics in yogurt:

  • They improve digestion and intestinal transit: They help break down food and make digestion easier, relieving discomfort such as acidity or bloating. They also regulate intestinal transit, helping with both constipation and diarrhea by balancing the gut flora. Many people report lighter, more regular digestion when consuming probiotic yogurt daily.
  • They maintain a balanced gut microbiota: Probiotics limit the growth of harmful bacteria in the gut, helping prevent gastrointestinal infections. Simply put, they act as “defenders” in the gut that stop harmful microbes like E. coli or Salmonella from multiplying and causing diarrhea or other digestive problems.
  • They strengthen the immune system: A large portion of our immune defenses resides in the intestine, and probiotics help strengthen them. A healthy gut flora is known to stimulate the body’s immune response. For example, certain probiotics increase the production of defensive cells (like macrophages), which can lead to fewer common infections such as colds or gastroenteritis.
  • They enhance nutrient absorption: The beneficial bacteria in yogurt help improve the absorption of certain vitamins and minerals. For instance, they aid in the assimilation of vitamin B, calcium, iron, and other essential nutrients. They also help digest lactose in people with mild intolerance, making it easier to consume dairy products.
  • They may benefit other areas of health: A balanced gut is associated with positive effects beyond digestion. Studies suggest that regularly consuming probiotics could be linked to a lower risk of certain intestinal diseases (like colitis or irritable bowel syndrome), improvements in some cases of allergies and eczema, and even positive effects on mood due to the gut-brain connection. While much research is still ongoing, maintaining a healthy microbiota appears to play a key role in overall wellness.

In short, probiotic yogurt is a powerful ally for digestive health: it protects and balances your gut flora, improves daily digestion, helps you absorb more nutrients from food, and strengthens your natural defenses. And all of that in a delicious, accessible food like yogurt!

Infographic showing the health benefits of probiotic yogurts including digestion, immunity, gut balance, and nutrient absorption

What is the best probiotic yogurt?

Plain, unsweetened yogurt made with milk and live cultures is considered the healthiest option. When it comes to the “best” probiotic yogurt, nutritionists agree that quality and composition matter more than the brand. A good probiotic yogurt should be as simple as possible: ideally plain, whole-milk, with no added sugars, made only from milk and live bacterial cultures. This type of traditional yogurt contains the necessary beneficial bacteria (like lactobacilli and bifidobacteria) without unnecessary additives like sugar, artificial sweeteners, colorants, or thickeners.

Why is plain yogurt usually the best?
Because without sugar or additives, its probiotic and nutritional benefits remain intact. Also, the natural fat in whole-milk yogurt is not harmful—on the contrary, it provides fat-soluble vitamins such as vitamin D, which are often lost in low-fat versions. Natural yogurt provides protein, calcium, and probiotics, and its neutral flavor makes it easy to pair with fresh fruit, honey, or cereals if you want a healthier touch of sweetness.

Plain Greek yogurt is also an excellent probiotic option. Greek yogurt is a strained yogurt that’s creamier and higher in protein, while still containing beneficial active cultures. It’s always best to choose it without added sugar or flavors to make the most of its benefits. According to experts, the healthiest options on the market are first, traditional plain yogurt, and second, plain Greek yogurt—both without added sweeteners or syrups.

In summary, the best probiotic yogurt will be one that meets these criteria: simple ingredients (milk and live cultures), no added sugar, and preferably whole milk. Many brands offer yogurts labeled as “bifidus” or “probiotic,” but it’s always important to check the label: it should clearly state that it contains live and active cultures (some labels say “with live cultures” or specify the bacterial strains) and it shouldn’t be packed with sugar. A good-quality plain or Greek yogurt—even without fancy marketing—is already an excellent daily probiotic. If you want more flavor, just add chopped fruit or a bit of natural sweetener to keep it healthy.

Are probiotic yogurts good for children?

Yes, probiotic yogurts can also be beneficial for children—with some considerations. Yogurt is often one of the first dairy products introduced during infancy (once complementary feeding has started, usually between 6 and 9 months, always under pediatric guidance). Natural yogurts contain cultures that help digest lactose, making them well tolerated by many babies and children who can also benefit from their properties.

Probiotics in children work similarly to how they do in adults: they help maintain a balanced gut microbiota and a healthy digestive system. They may help prevent or reduce common childhood digestive issues such as diarrhea (including antibiotic-associated diarrhea), constipation, and colic. They also support the immune system: some studies suggest a slight reduction in the frequency of respiratory infections (like colds or ear infections) by improving gut defenses.

It’s important to choose yogurts appropriate for the child’s age. For young children, it’s best to offer whole-milk natural yogurt without added sugar (the milk fat is beneficial for their development). You can naturally sweeten it with mashed fruit if they prefer a sweeter taste. There are also yogurt products made especially for children, such as petit-suisse types (like Danonino) and child-friendly probiotic yogurts, usually sold in small portions with appealing flavors. These versions are safe and designed for young kids, though it’s important to keep an eye on the sugar content in commercial varieties.

Brands like Danimals (the U.S. version of Danonino) and drinkable yogurts for kids contain probiotics and are quite popular. Even classic yogurt tubes or pouches (like Yoplait Go-Gurt) contain live cultures. In general, offering a child one yogurt a day can be an easy way to improve their digestive health while providing calcium and protein.

As always, if there are specific concerns—such as a compromised immune system or a medical condition—it’s best to consult a pediatrician. But for the majority of healthy children, including probiotic yogurts in their diet is safe and beneficial, helping to establish healthy eating habits from an early age. In fact, many manufacturers indicate that their probiotic yogurts are suitable for the whole family, including children, as part of a balanced diet.

What types of probiotic strains are there and what are they used for?

When we talk about probiotics, we’re not referring to just one type of bacteria. There are many different probiotic strains, each with its own unique properties. Just like not all medications treat the same condition, not all probiotics serve the same purpose in the body. Understanding the most common strains and their benefits can help you choose the best yogurt or supplement depending on your needs—whether it’s improving digestion, boosting immunity, or relieving gas.

Below is a summary of the most widely used probiotic strains in yogurts and fermented products, along with what they do:

Table showing different probiotic strains, their benefits, recommended uses, and food sources

Can probiotic yogurts relieve constipation and irritable bowel syndrome (IBS)?

Many people consume probiotic yogurts looking for relief from specific digestive issues such as chronic constipation or irritable bowel syndrome (IBS). But what does the evidence say?

  • Probiotics and constipation: Several studies suggest that certain probiotic strains help improve bowel regularity. In people with chronic functional constipation (that is, long-standing constipation without an apparent organic cause), probiotics have been shown to reduce intestinal transit time (meaning they speed up how long it takes stool to move through the colon) and improve stool consistency and frequency.
    In particular, strains like Bifidobacterium lactis stand out as allies against constipation: they’ve been shown to increase the number of beneficial bifidobacteria in the colon, resulting in more regular and easier bowel movements. Other bacteria such as Lactobacillus casei and L. reuteri have also demonstrated positive effects in clinical studies, helping relieve constipation symptoms.

    Many probiotic yogurts (such as Activia by Danone) contain these specific strains designed to improve gut transit. In fact, daily consumption of bifidus yogurts is popularly associated with “helping you go to the bathroom more regularly,” which is also backed by science—at least in mild cases.Of course, every body is different, but including a probiotic yogurt in your diet, along with enough fiber and water, can be a useful and natural way to regulate your bowel movements.

  • Probiotics and irritable bowel syndrome (IBS): IBS is a common digestive disorder characterized by abdominal pain, bloating, gas, and irregular bowel habits (sometimes diarrhea, sometimes constipation, or both alternating). There is no definitive cure, but diet and microbiota play a major role in its symptoms. In this context, probiotics may offer support to some individuals with IBS. Several studies and meta-analyses have concluded that certain probiotics bring modest yet significant improvements in IBS symptoms like abdominal pain and bloating, as well as reduced flatulence.
    For example, strains of Bifidobacterium (breve, longum, infantis) and Lactobacillus (acidophilus, casei, plantarum) have been linked to reduced discomfort and less gas in IBS patients.
    All yogurts contain live Lactobacillus, and some also include Bifidobacterium, so they could contribute to those improvements. While not a magic cure (IBS requires a broader approach—such as FODMAP diet in some cases, stress management, etc.), eating probiotic yogurt daily may help reduce symptoms in some people. It’s a simple, safe option that can complement other gut care strategies.

In short, probiotic yogurts can be helpful both for relieving constipation and for improving certain symptoms of IBS, thanks to their regulating effect on gut flora and intestinal function. Their effects vary depending on the person and the specific probiotic strain, but scientific evidence supports their use as a complementary tool in managing digestive issues. If you suffer from chronic constipation or IBS, adding a probiotic yogurt to your daily routine is a simple step that could bring relief—especially when combined with a balanced diet and guidance from your doctor or nutritionist.

What brands of probiotic yogurts are recommended?

There is a wide variety of yogurts enriched with probiotics on the market. Below, we list some popular probiotic yogurt brands both in Spain and internationally, along with their characteristics, to help you choose the one that best suits you or your family:

Danone
Yoplait
Chobani
Fage
Bífidus

In summary, the best probiotic yogurt brands are those that offer products with a high count of live cultures and low sugar content. Activia is ideal if your goal is to improve digestive regularity; Chobani or Fage if you’re looking for a dense, high-protein yogurt with diverse strains; Yoplait or traditional yogurts if you want something for the whole family and easy to find; and options like Yakult or Actimel if you prefer a drinkable format to support your immunity or microbiota. You have many options to try and see which works best for you and your family!

Conclusion: Dare to take care of your digestive health with a yogurt a day

We’ve seen that their benefits range from improving daily digestion and gut flora to potentially boosting immunity and relieving certain digestive problems. Moreover, there are options for all tastes and ages—from children to older adults—across multiple brands and formats.

Something many people don’t know is that the probiotics we consume in yogurts are made thanks to biotechnology, specifically in bioreactors. These systems make it possible to grow beneficial bacterial strains in a controlled and safe way. Specialized companies like TECNIC, leaders in bioprocess solutions, manufacture bioreactors designed to produce microorganisms such as Lactobacillus and Bifidobacterium, which are found in probiotic yogurts. So, what ends up in your fridge starts much earlier in a highly technical and sterile environment, where science and technology work together to improve your digestive well-being.

As a final recommendation, remember to maintain a balanced and varied diet. Probiotics work best when part of an eating pattern rich in fiber, fruits, vegetables, and proper hydration. If you’re unsure which yogurt is right for you, or if you have a specific health condition, talk to a doctor or nutritionist. We hope this guide has answered your questions! Next time you're at the grocery store in front of the dairy section, you’ll know exactly what to look for.

Probiotic Yogurts FAQ

Frequently Asked Questions (FAQ)

1. What are the benefits of probiotic yogurts?

Probiotic yogurts support digestion, improve nutrient absorption, and help maintain a healthy gut microbiota.

2. Is it okay to eat probiotic yogurt every day?

Yes, daily consumption of probiotic yogurt is generally safe and may improve gut health and immune function over time.

3. Which yogurt has the most probiotics?

Yogurts labeled with live and active cultures, especially those with Lactobacillus and Bifidobacterium strains, tend to have the highest probiotic content.

4. Are probiotic yogurts good for kids?

Yes, probiotic yogurts can support digestive health and immunity in children when consumed as part of a balanced diet.

5. Can you eat probiotic yogurt at night?

Yes, eating probiotic yogurt at night is safe and may support overnight digestion and gut function.

6. How do I know if probiotics are working?

Signs include improved digestion, reduced bloating, better bowel movements and overall gut comfort.

7. Are Greek yogurts probiotic?

Some Greek yogurts contain probiotics, but it's important to check the label for live and active cultures.

References

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We understand the importance of flexibility and efficiency in laboratory processes. That's why our equipment is designed to be compatible with Cassette filters, an advanced solution for a variety of filtration applications. Although we do not manufacture the filters directly, our systems are optimized to take full advantage of the benefits that Cassette filters offer.

Cassette filters are known for their high filtration capacity and efficiency in separation, making them ideal for ultrafiltration, microfiltration, and nanofiltration applications. By integrating these filters into our equipment, we facilitate faster and more effective processes, ensuring high-quality results.

Our equipment, being compatible with Cassette filters, offers greater versatility and adaptability. This means you can choose the filter that best suits your specific needs, ensuring that each experiment or production process is carried out with maximum efficiency and precision.

Moreover, our equipment stands out for its 100% automation capabilities. Utilizing advanced proportional valves, we ensure precise control over differential pressure, transmembrane pressure, and flow rate. This automation not only enhances the efficiency and accuracy of the filtration process but also significantly reduces manual intervention, making our systems highly reliable and user-friendly.

Hollow Fiber

We recognize the crucial role of flexibility and efficiency in laboratory processes. That's why our equipment is meticulously designed to be compatible with Hollow Fiber filters, providing an advanced solution for a broad spectrum of filtration applications. While we don't directly manufacture these filters, our systems are finely tuned to harness the full potential of Hollow Fiber filters.

Hollow Fiber filters are renowned for their exceptional performance in terms of filtration efficiency and capacity. They are particularly effective for applications requiring gentle handling of samples, such as in cell culture and sensitive biomolecular processes. By integrating these filters with our equipment, we enable more efficient, faster, and higher-quality filtration processes.

What sets our equipment apart is its 100% automation capability. Through the use of sophisticated proportional valves, our systems achieve meticulous control over differential pressure, transmembrane pressure, and flow rate. This level of automation not only boosts the efficiency and precision of the filtration process but also significantly diminishes the need for manual oversight, rendering our systems exceptionally reliable and user-friendly.

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Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Microbial configuration

The microbial configuration of the eLab Advanced is equipped with a Rushton turbine specifically designed for high-oxygen-demand processes such as bacterial and yeast fermentations. The radial-flow impeller generates strong mixing and intense gas dispersion, promoting high oxygen transfer rates and fast homogenization of nutrients, antifoam and pH control agents throughout the vessel. This makes it particularly suitable for robust microbial strains operating at elevated agitation speeds and aeration rates.

Operators can adjust agitation and gas flow to reach the required kLa while maintaining consistent mixing times, even at high cell densities. This configuration is an excellent option for users who need a powerful, reliable platform to develop and optimize microbial processes before transferring them to pilot or production scales.

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Technical specifications

Materials and finishes

Typical
  • Product-contact parts: AISI 316L (1.4404), typical Ra < 0.4 µm (16 µin)
  • Non-contact parts/skid: AISI 304/304L
  • Seals/elastomers: platinum-cured silicone, EPDM and/or PTFE (material set depends on selection)
  • Elastomers compliance (depending on selected materials): FDA 21 CFR 177.2600 and USP Class VI
  • Surface treatments: degreasing, pickling and passivation (ASTM A380 and ASTM A968)
  • Roughness control on product-contact surfaces

Design conditions

Pressure & temperature

Defined considering non-hazardous process fluids (PED group 2) and jacket steam/superheated water (PED group 5), depending on configuration and project scope.

Reference design envelope
ModeElementWorking pressure (bar[g])Working pressure (psi[g])T max (°C / °F)
ProcessVessel0 / +2.50 / +36.3+90 / 194
ProcessJacket0 / +3.80 / +55.1+90 / 194
SterilisationVessel0 / +2.50 / +36.3+130 / 266
SterilisationJacket0 / +3.80 / +55.1+150 / 302
Jacket working pressure may also be specified as 0 / +4 bar(g) (0 / +58.0 psi[g]) depending on design selection; final values are confirmed per project.

Pressure control and safeguards

Typical
  • Designed to maintain a vessel pressure set-point typically in the range 0 to 2.5 bar(g)
  • Aseptic operation commonly around 0.2 to 0.5 bar(g) to keep the vessel slightly pressurised
  • Overpressure/underpressure safeguards included per configuration and regulations
  • Pressure safety device (e.g., rupture disc and/or safety valve) included according to configuration

Agitation

Reference ranges
Working volumeMU (Cell culture), referenceMB (Microbial), reference
10 L0 to 300 rpm0 to 1000 rpm
20 L0 to 250 rpm0 to 1000 rpm
30 L0 to 200 rpm0 to 1000 rpm
50 L0 to 180 rpm0 to 1000 rpm

Integrated peristaltic pumps (additions)

Typical

The equipment typically includes 4 integrated variable-speed peristaltic pumps for sterile additions (acid/base/antifoam/feeds). Actual flow depends on selected tubing and calibration.

ParameterTypical valueNotes
Quantity4 units (integrated)In control tower; assignment defined by configuration
Speed0-300 rpmVariable control from eSCADA
Minimum flow0-10 mL/minExample with 0.8 mm ID tubing; depends on tubing and calibration
Maximum flowUp to ~366 mL/minExample with 4.8 mm ID tubing; actual flow depends on calibration
Operating modesOFF / AUTO / MANUAL / PROFILEAUTO typically associated to pH/DO/foam loops or recipe
FunctionsPURGE, calibration, totaliser, PWMPWM available for low flow setpoints below minimum operating level

Gas flow control (microbial reference capacity)

Reference

For microbial culture (MB), gas flow controllers (MFC) are typically sized based on VVM targets. Typical reference VVM range: 0.5-1.5 (to be confirmed by process).

Working volume (L)VVM minVVM maxAir (L/min)O2 (10%) (L/min)CO2 (20%) (L/min)N2 (10%) (L/min)
100.51.55-150.5-1.51-30.5-1.5
200.51.510-301-32-61-3
300.51.515-451.5-4.53-91.5-4.5
500.51.525-752.5-7.55-152.5-7.5
O2/CO2/N2 values are shown as reference capacities for typical gas blending strategies (10% O2, 20% CO2, 10% N2). Final gas list and ranges depend on process and configuration.

Instrumentation and sensors

Typical

Instrumentation is configurable. The following list describes typical sensors integrated in standard configurations, plus common optional PAT sensors.

Variable / functionTypical technology / interfaceStatus (STD/OPT)
Temperature (process/jacket)Pt100 class A RTDSTD
Pressure (vessel/lines)Pressure transmitter (4-20 mA / digital)STD
Level (working volume)Adjustable probeSTD
pHDigital pH sensor (ARC or equivalent)STD
DO (pO2)Digital optical DO sensor (ARC or equivalent)STD
FoamConductive/capacitive foam sensorSTD
Weight / mass balanceLoad cell (integrated in skid)STD
pCO2Digital pCO2 sensor (ARC or equivalent)OPT
Biomass (permittivity)In-line or in-vessel sensorOPT
VCD / TCDIn-situ cell density sensorsOPT (MU)
Off-gas (O2/CO2)Gas analyser for OUR/CEROPT
ORP / RedoxDigital ORPOPT
Glucose / LactatePAT sensorOPT

Automation, software and connectivity

Typical

The platform incorporates TECNIC eSCADA (typically eSCADA Advanced for ePILOT) to operate actuators and control loops, execute recipes and manage process data.

Main software functions
  • Main overview screen with process parameters and trends
  • Alarm management (real-time alarms and historical log) with acknowledgement and comment option
  • Manual/automatic modes for actuators and control loops
  • Recipe management with phases and transitions; parameter profiles (multi-step) for pumps and setpoints
  • Data logging with configurable period and export to CSV; PDF report generation
Common control loops
  • Temperature control (jacket heating/cooling)
  • Pressure control (headspace) with associated valve management
  • pH control via acid/base addition pumps and optional CO2 strategy
  • DO control with cascade strategies (agitation, air, O2, N2) depending on package and configuration
  • Foam control (foam sensor and automatic antifoam addition)
Data integrity and 21 CFR Part 11

Support for 21 CFR Part 11 / EU GMP Annex 11 is configuration- and project-dependent and requires customer procedures and validation (CSV).

Utilities

Reference

Utilities depend on final configuration (e.g., AutoSIP vs External SIP) and destination market (EU vs North America). The following values are typical reference points.

UtilityTypical service / configurationPressureFlow / powerNotes
ElectricalEU base: 400 VAC / 50 Hz (3~)N/AAutoSIP: 12 kW; External SIP: 5 kWNA option: 480 VAC / 60 Hz; cabinet/wiring per NEC/NFPA 70; UL/CSA as required
Process gasesAir / O2 / CO2 / N2Up to 2.5 bar(g) (36.3 psi)According to setpointTypical OD10 pneumatic connections; final list depends on package
Instrument airPneumatic valvesUp to 6 bar(g) (87.0 psi)N/ADry/filtered air recommended
Cooling waterJacket cooling water2 bar(g) (29.0 psi)25 L/min (6.6 gpm)6-10 °C (43-50 °F) typical
Cooling waterCondenser cooling water2 bar(g) (29.0 psi)1 L/min (0.26 gpm)6-10 °C (43-50 °F) typical
Steam (External SIP)Industrial steam2-3 bar(g) (29.0-43.5 psi)30 kg/h (66 lb/h)For SIP sequences
Steam (External SIP)Clean steam1.5 bar(g) (21.8 psi)8 kg/h (18 lb/h)Depending on plant strategy

Compliance and deliverables

Typical

Depending on destination and project scope, the regulatory basis may include European Directives (CE) and/or North American codes. The exact list is confirmed per project and stated in the Declaration(s) of Conformity when applicable.

ScopeEU (typical references)North America (typical references)
Pressure equipmentPED 2014/68/EUASME BPVC Section VIII (where applicable)
Hygienic designHygienic design good practicesASME BPE (reference for bioprocessing)
Machine safetyMachinery: 2006/42/EC (until 13/01/2027) / (EU) 2023/1230OSHA expectations; NFPA 79 (industrial machinery) - project dependent
Electrical / EMCLVD 2014/35/EU; EMC 2014/30/EUNEC/NFPA 70; UL/CSA components and marking as required
Materials contactEC 1935/2004 + EC 2023/2006 (GMP for materials) where applicableFDA 21 CFR (e.g., 177.2600 for elastomers) - materials compliance
Software / CSVEU GMP Annex 11 (if applicable)21 CFR Part 11 (if applicable)
Standard documentation package
  • User manual and basic operating instructions
  • P&ID / layout drawings as per project scope
  • Material certificates and finish/treatment certificates (scope dependent)
  • FAT report (if included in contract)
Optional qualification and commissioning services
  • SAT (Site Acceptance Test)
  • IQ / OQ documentation and/or execution (scope agreed with customer)
  • CSV support package for regulated environments (ALCOA+ considerations, backups, time synchronisation, etc.)

Ordering and configuration

Project-based

ePILOT BR is configured per project. To define the right MU/MB package, volumes and options (utilities, sensors, software and compliance), please contact TECNIC with your URS or request the configuration questionnaire.

The information provided above is for general reference only and may be modified, updated or discontinued at any time without prior notice. Values and specifications are indicative and may vary depending on project scope, configuration and applicable requirements. This content does not constitute a binding offer, warranty, or contractual commitment. Any final specifications, deliverables and acceptance criteria will be confirmed in the corresponding quotation, technical documentation and/or contract documents.

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Technical specifications

    ePILOT BR configuration questionnaire









    Project details



















    FAT:

    Shipment:

    Installation:

    SAT:

    IQ/OQ:


    Process and automation requirements























    MU only (cell culture)


    MB only (microbial)


    Utilities and infrastructure



    North America specific















    Connections, consumables and compliance












    EU specific




    North America specific


    Software / CSV (GMP)


    Validation, testing and documentation










    GMP / CSV


    Logistics and installation











    Additional comments




    Cellular configuration

    The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

    Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

    Technical specifications

    Models and working volumes

    Tank

    The ePlus Mixer platform combines an ePlus Mixer control tower with Tank frames and eBag 3D consumables. Tank can be supplied in square or cylindrical configurations (depending on project) to match the bag format.

    Tank modelNominal volumeMinimum volume to start agitation*
    Tank 50 L50 L15 L
    Tank 100 L100 L20 L
    Tank 200 L200 L30 L
    Tank 500 L500 L55 L
    *Values based on agitation start interlocks per tank model. Final performance depends on the selected eBag 3D, fluid properties and configuration.

    Design conditions and operating limits

    Reference

    Reference limits are defined for the ePlus Mixer and the Tank. It is recommended to validate the specific limits of the selected eBag 3D and single-use sensors for the customer’s process.

    ElementOperating pressureMaximum pressure (safety)Maximum working temperature
    ePlus Mixer (control tower)ATM0.5 bar(g)90 °C
    TankATM0.5 bar(g)45 °C
    Jacket (if applicable)N/A1.5 barDepends on utilities / scope
    The 0.5 bar(g) limit is associated with the equipment design, the circuit is protected by a safety valve. Confirm final limits on the equipment nameplate and project specification.

    Materials and finishes

    Typical
    • Control tower housing and frame: stainless steel 304
    • Product-contact metallic hard parts (if applicable): stainless steel 316 (defined in project manufacturing documentation)
    • Non-product-contact metallic parts: stainless steel 304
    • eBag consumable: single-use polymer (supplier dependent, gamma irradiation / sterilisation per specification)
    • Vent filters: PP (polypropylene), per component list
    For GMP projects, the recommended documentation package includes material certificates, surface finish certificates (Ra if applicable) and consumable sterility/irradiation certificates.

    Agitation system

    Magnetic

    Non-invasive magnetic agitation, the impeller is integrated in the eBag 3D Mixer format, avoiding mechanical seals. Agitation speed is controlled from the HMI, with start interlocks linked to the tank model and minimum volume.

    Reference speed range
    • Typical agitation range: 120 to 300 rpm (configuration dependent)
    • Magnetic drive motor (reference): Sterimixer SMA 85/140, 50 Hz, 230/400 V, 0.18 kW
    • Gear reduction (reference): 1:5
    • Actuation (reference): linear actuator LEYG25MA, stroke 30–300 mm, speed 18–500 mm/s (for positioning)
    Final rpm and mixing performance depend on tank size, bag format and process requirements.

    Weighing and volume control

    Integrated

    Weight and derived volume control are performed using 4 load cells integrated in the tank frame legs and a weight indicator. Tare functions are managed from the HMI to support preparation steps and additions by mass.

    ComponentReference modelKey parameters
    Load cells (x4)Mettler Toledo SWB505 (stainless steel)550 kg each, output 2 mV/V, IP66
    Weight indicatorMettler Toledo IND360 DINAcquisition and HMI display, tare and “clear last tare”
    For installation engineering, total floor load should consider product mass + equipment mass + margin (recommended ≥ 20%).

    Pumps and fluid handling

    Standard

    The platform includes integrated pumps for additions and circulation. Final tubing selection and calibration define the usable flow range.

    Included pumps (reference)
    • 3 integrated peristaltic pumps for additions (acid/base/media), with speed control from HMI
    • 1 integrated centrifugal pump for circulation / transfer (DN25)
    Peristaltic pumps (reference)
    ParameterReferenceNotes
    Quantity3 unitsIntegrated in the control tower
    Pump headHYB101 (Hygiaflex)Example tubing: ID 4.8 mm, wall 1.6 mm
    Max speed300 rpmSpeed control reference: 0–5 V
    Max flow (example)365.69 mL/minDepends on tubing and calibration
    Centrifugal pump (reference)
    ParameterReference
    ModelEBARA MR S DN25
    Power0.75 kW
    FlowUp to 42 L/min
    PressureUp to 1 bar
    For circulation and sensor loops, the eBag 3D format can include dedicated ports (depending on the selected consumable and application).

    Thermal management (optional jacket)

    Optional

    Tank can be supplied with a jacket (single or double jacket options). The thermal circuit includes control elements and a heat exchanger, enabling temperature conditioning depending on utilities and project scope.

    • Jacket maximum pressure (reference): 1.5 bar
    • Thermal circuit safety: pressure regulator and safety valve (reference set-point 0.5 bar(g))
    • Heat exchanger (reference): T5-BFG, 12 plates, alloy 316, 0.5 mm, NBRP
    • Solenoid valves (reference): SMC VXZ262LGK, 1", DC 24 V, 10.5 W
    • Jacket sequences: fill / empty / flush (scope dependent)
    The tank maximum temperature may depend on the thermal circuit and consumable limits. Confirm final values with the selected eBag 3D specification.

    Instrumentation and sensors

    Optional SU

    Single-use sensors can be integrated via dedicated modules. The following references describe typical sensors and interfaces listed in the datasheet.

    VariableReference modelInterface / protocolSupplyOperating temperatureIP
    pHOneFerm Arc pH VP 70 NTC (SU)Arc Module SU pH, Modbus RTU7–30 VDC5–50 °CIP67
    ConductivityConducell-P SU (SU)Arc Module Cond-P SU, Modbus RTU7–30 VDC0–60 °CIP64
    TemperaturePt100 ø4 × 52 mm, M8 (non-invasive)Analog / acquisition moduleProject dependentProject dependentProject dependent
    Measurement ranges and final sensor list depend on the selected single-use components and project scope.

    Automation, software and data

    Standard + options

    The ePlus SUM control tower integrates an industrial PLC and touch HMI. Standard operation supports Manual / Automatic / Profile modes, with optional recipe execution depending on selected software scope.

    Software scope (reference)
    • Standard: eBASIC (base HMI functions)
    • Optional: eSCADA Basic or eSCADA Advanced (project dependent)
    • Trends, alarms and profiles, profiles up to 100 steps (depending on scope)
    • Data retention (reference): up to 1 year
    Connectivity (reference)
    • Industrial Ethernet and integrated OPC server (included)
    • Remote access option (project dependent)

    Utilities and facility interfaces

    Typical

    Installation requirements depend on jacket and temperature scope and the customer layout. The following values are typical references.

    UtilityPressureFlowConnectionsNotes
    Electrical supplyN/AReference: 18 A380–400 VAC, 3~ + N, 50 HzConfirm per final configuration and destination market
    EthernetN/AN/ARJ45OPC server, LAN integration
    Tap water2.5 barN/A1/2" (hose connection)Jacket fill and services, tank volume about 25 L
    Cooling water2–4 bar10–20 L/min2 × 3/4" (hose connection)Heat exchanger and jacket cooling
    Process air2–4 barN/A1/2" quick couplingUsed for jacket emptying
    DrainN/AN/A2 × 3/4" (hose connection)For draining
    ExhaustN/AN/AN/AOptional (depending on project)
    Stack light (optional)N/AN/AN/A3-colour indication, as per scope
    During FAT, verify in the installation checklist that the available utilities match the selected configuration and scope.

    Documentation and deliverables

    Project-based

    Deliverables depend on scope and project requirements. The following items are typical references included in the technical documentation package.

    • Datasheet and user manual (HMI and system operation)
    • Electrical schematics, PLC program and backup package (scope dependent)
    • P&ID, layout and GA drawings (PDF and/or CAD formats, project dependent)
    • Factory Acceptance Test (FAT) protocol and FAT report (as per contract)
    • Installation checklist
    • Material and consumable certificates, as required for regulated projects (scope dependent)
    On-site services (SAT, IQ/OQ) and extended compliance packages are optional and defined per project.

    Ordering and configuration

    Contact

    The ePlus Mixer scope is defined per project. To select the right tank size, bag format, sensors and optional jacket and software, please share your URS or request the configuration questionnaire.

    The information provided above is for general reference only and may be modified, updated or discontinued at any time without prior notice. Values and specifications are indicative and may vary depending on project scope, configuration and applicable requirements. This content does not constitute a binding offer, warranty, or contractual commitment. Any final specifications, deliverables and acceptance criteria will be confirmed in the corresponding quotation, technical documentation and/or contract documents.

    Cellular configuration

    The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

    Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

    Scale

    Bioreactors engineered for smooth scale-up

    From S to XL, with a clear scale path

    Move from laboratory to pilot and production with a structured range: eLab (0.5–10 L), ePilot (30–50 L), eProd (100–2000 L). Scale with clearer continuity across platforms, supporting the same key control priorities and configuration paths for a smoother transition between volumes.